➢ Product Name: Magigen Thermostable RNase HII Kit | Ribonuclease RNase H2 Kit
➢ Catalog#: A006S
➢ Size: 100pmol
➢ RNase HII characteristics and functions
Magigen Thermostable RNase HII | Ribonuclease RNase H2 is an endoribonuclease that specially cleaves 5´ end to a ribonucleotide within the context of a double stranded DNA.
Magigen Thermostable RNase HII leaves 3´ hydroxyl and 5´ phosphate ends. RNase H2 will also cleave at multiple sites along the RNA portion of an Okazaki fragment.
The Magigen thermostable RNase HII enzyme, derived from an artificially engineered thermophilus rnhb gene with a molecular weight of 36 KD and a C-end carrying a histidine tag (his tag), has a wider temperature applicable region as well as more rapid nicking efficiency than E.coli RNase HII and wild-type Tth RNase H2, and can be used for rhPCR reactions.
➢ Contents
| Components | Volume |
| 10XThermoPol Buffer 6 | 1ml x 1 |
| Thermostable RNase HII ( 5U/μl) | 50μl x 1 |
| 1× Dilution Buffer 4 ( 5U/μl) | 50μl x 1 |
➢ Unit Definition
One unit is defined as the amount of enzyme required to yield a fluorescence signal consistent with the nicking of 100 pmol of synthetic double-stranded RNA substrate containing a single ribonucleotide near the quencher of a fluorophore/quencher pair in 15 minutes at 60°C in 1X ThermoPol Buffer.
➢ Storage Conditions and Shelf Life
Sealed and stored at -20 ℃, 12 months.
➢ How to use RNase HII?
Reagent Test Control (N) Vol (μL)
10× Reaction Buffer 6 1× 1× 2.5
RNaseH probe 200 nM 200 nM 1
RNase HⅡ 2.5U 2.5U 0.5
template Tm 59.5℃ 20 nM -- 0.5
Total Vol（add H2O） 25
60℃，60min。
RNase HII Test Data
Fig1. Magigen RNase HII Test VS Control N
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